ARG58912
anti-ALT / Alanine Transaminase antibody
anti-ALT / Alanine Transaminase antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes ALT / Alanine Transaminase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALT / Alanine Transaminase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human ALT / Alanine Transaminase. (NP_005300.1) (KLTEQVFNEAPGISC) |
| Conjugation | Un-conjugated |
| Alternate Names | Glutamate pyruvate transaminase 1; AAT1; GPT1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Alanine aminotransferase 1; ALT1; EC 2.6.1.2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 52 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GPT |
| Gene Full Name | glutamic-pyruvate transaminase (alanine aminotransferase) |
| Background | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| Function | Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 55 kDa |
Images (1) Click the Picture to Zoom In
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ARG58912 anti-ALT / Alanine Transaminase antibody WB image
Western blot: 35 µg of Human liver lysate (in RIPA buffer) stained with ARG58912 anti-ALT / Alanine Transaminase antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
