ARG65169
anti-ACO2 / Aconitase 2 antibody
anti-ACO2 / Aconitase 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ACO2 / Aconitase 2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACO2 / Aconitase 2 |
| Antigen Species | Human |
| Immunogen | C-QHVDVSPTSQRLQ |
| Conjugation | Un-conjugated |
| Alternate Names | Aconitase; ACONM; EC 4.2.1.3; ICRD; Aconitate hydratase, mitochondrial; Citrate hydro-lyase; OCA8; HEL-S-284 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99798 Human Aconitate hydratase, mitochondrial |
|---|---|
| Background | The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: Aconitase antibodies; Aconitase ELISA Kits; Anti-Goat IgG secondary antibodies; Related news: TCA intermediate fumarate promotes mitobiogenesis |
| Research Area | Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 85 kDa |
Images (2) Click the Picture to Zoom In
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ARG65169 anti-ACO2 / Aconitase 2 antibody WB image
Western blot: Heart lysate (35 µg protein in RIPA buffer) stained with ARG65169 anti-ACO2 / Aconitase 2 antibody at 0.01 µg/ml dilution.
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ARG65169 anti-ACO2 / Aconitase 2 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG65169 anti-ACO2 / Aconitase 2 antibody at 3.8 µg/ml dilution followed by AP-staining.
