ARG82192
Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)
Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species
Overview
| Product Description | ARG82192 Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Sorbitol Dehydrogenase activity in serum, plasma, urine, tissue and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Sorbitol Dehydrogenase |
| Conjugation Note | Read at 565 nm |
| Sensitivity | 0.1 U/L |
| Detection Range | 0.1 - 125 U/L |
| Sample Type | serum, plasma, urine, tissue and cell culture supernatants |
| Sample Volume | 20 µl |
| Alternate Names | SORD1; L-iditol 2-dehydrogenase; Sorbitol dehydrogenase; HEL-S-95n; EC 1.1.1.14 |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 15 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SORD |
| Gene Full Name | sorbitol dehydrogenase |
| Background | Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010] |
| Function | Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. [UniProt] |
| Cellular Localization | Mitochondrion membrane; Peripheral membrane protein. Cell projection, cilium, flagellum. Note=Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82192 Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 125 U/L Sorbitol Dehydrogenase reaction, using ARG82192 Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric).
| Title | Download Link |
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| ARG82192 Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) User manual |
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