ARG21616

Rat anti-Mouse IgA antibody [11-44-2]

Rat anti-Mouse IgA antibody [11-44-2] for ELISA,Flow cytometry,FLISA,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

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Overview

Product Description Rat Monoclonal antibody [11-44-2] recognizes Mouse IgA
Tested Reactivity Ms
Tested Application ELISA, FACS, FLISA, IHC-Fr, IHC-P, WB
Host Rat
Clonality Monoclonal
Clone 11-44-2
Isotype IgG1, kappa
Target Name IgA
Antigen Species Mouse
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1 - 0.05 μg/ml
FACS0.2 - 1 μg/10^6 cells
FLISAAssay-dependent
IHC-Fr2 - 5 μg/ml
IHC-P2 - 10 μg/ml
WBAssay-dependent
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Boil tissue section in Citrate buffer (pH 6.0) for 20 min. The tissue section was stained with ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) at 2 - 10 µg/ml dilution followed by an HRP-conjugated secondary antibody, DAB and hematoxylin. Control (left).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] ELISA image

    ELISA: The plate was coated with purified Mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21616 Rat anti-Mouse IgA antibody [11-44-2] followed by ARG21696 Mouse anti-Rat IgG1 antibody [G17E7] (HRP).

  • ARG21616 Rat anti-Mouse IgA antibody [11-44-2] IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse intestine tissue stained with ARG20689 Rat IgG1 Isotype Control antibody [KLH/G1-2-2] (left) and ARG21616 Rat anti-Mouse IgA antibody [11-44-2] (right) (2-5 μg/ml) followed by ARG21695 Mouse anti-Rat IgG1 antibody [G17E7] (FITC) and DAPI.

Specific References

Weissella paramesenteroides WpK4 ameliorate the experimental amoebic colitis by increasing the expression of MUC-2 and the intestinal epithelial regeneration.

IHC-P / Mouse

Prado GKS et al.
J Appl Microbiol.,  (2020)

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