ARG82095
Rat WFDC2 / HE4 ELISA Kit
Rat WFDC2 / HE4 ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82095-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82095-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82095-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82095-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82095-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82095-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82095-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82095-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82095-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82095-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82095-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82095 Rat WFDC2 / HE4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat WFDC2 / HE4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | WFDC2 / HE4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7%; Inter-Assay CV: 7.3% |
| Alternate Names | HE4; dJ461P17.6; WAP5; Putative protease inhibitor WAP5; Major epididymis-specific protein E4; WAP four-disulfide core domain protein 2; Epididymal secretory protein E4; EDDM4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8CHN3 Rat WAP four-disulfide core domain protein 2 |
|---|---|
| Gene Symbol | WFDC2 |
| Gene Full Name | WAP four-disulfide core domain 2 |
| Background | This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008] |
| Function | Broad range protease inhibitor. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: WFDC2 antibodies; WFDC2 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82095 Rat WFDC2 / HE4 ELISA Kit standard curve image
ARG82095 Rat WFDC2 / HE4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82095 Rat WFDC2 / HE4 ELISA Kit User's manual |
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