ARG82316
Rat OPN / Osteopontin ELISA Kit
Rat OPN / Osteopontin ELISA Kit for ELISA and Rat
Overview
| Product Description | ARG82316 Rat OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat OPN / Osteopontin in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | Not react with following recombinant Rat proteins: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HSP27, HGF, IL1 beta, IL1RA, IL2, IL4, IL5, sIL6R, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, MMP9, IL2R, PDGF, serpin E1, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TLR9, TNF alpha, TNF RI, TNF RII, VEGF and VEGF R1. |
| Target Name | OPN / Osteopontin |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5% Inter-Assay CV: 10% |
| Alternate Names | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SPP1 |
|---|---|
| Gene Full Name | secreted phosphoprotein 1 |
| Background | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: OPN antibodies; OPN ELISA Kits; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82316 Rat OPN / Osteopontin ELISA Kit standard curve image
ARG82316 Rat OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82316 Rat OPN / Osteopontin ELISA kit-User manual |
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