ARG81355

Rat FGF basic ELISA Kit

Rat FGF basic ELISA Kit for ELISA and Rat

Overview

Product Description ARG81355 Rat FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat FGF basic in serum or plasma.
Tested Reactivity Rat
Tested Application ELISA
Specificity The following recombinant Rat proteins prepared at 10 ng/ml were tested and exhibited no crossreactivity or interference: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HSP27, HGF, IL-1 beta, IL-1RA, IL-2, IL-4, IL-5, sFGF BASICR, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17C, IL-21, IL-23, IFNγ, MMP-2, MMP9, IL2R, PDGF, serpin E1, TGFβ1, TGFβ2, TGFβ3, TLR1, TLR2, TLR3, TLR9, TNF-α, TNF RI, TNF RII, VEGF, VEGF R1.
Target Name FGF basic
Conjugation HRP
Conjugation Note Read at 450 nm.
Sensitivity 3 pg/ml
Sample Type Serum or plasma.
Standard Range 15.63 - 1000 pg/ml
Sample Volume 100 µl
Alternate Names FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor

Application Instructions

Assay Time ~ 3 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54250 Rat FGF2

Swiss-port # P13109 Rat Fibroblast growth factor 2

Gene Symbol FGF2
Gene Full Name fibroblast growth factor 2 (basic)
Background The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
Highlight Related products:
FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins;
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PTM Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.

Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG81355 Rat FGF basic ELISA Kit standard curve image

    ARG81355 Rat FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81355 Rat FGF basic ELISA Kit User manual download icon Download