ARG81782
Rat CD62L / L-Selectin ELISA Kit
Rat CD62L / L-Selectin ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81782-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81782-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81782-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81782-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81782-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81782-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81782-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81782-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81782-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81782-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81782-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81782 Rat CD62L / L-Selectin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat soluble CD62L / L-Selectin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD62L / L-Selectin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 312.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 625 - 40000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.3%; Inter-Assay CV: 6.0% |
| Alternate Names | Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; CD antigen CD62L; PLNHR; LSEL; CD62L; Leukocyte-endothelial cell adhesion molecule 1; L-selectin; LAM1; LNHR; TQ1; CD62 antigen-like family member L; gp90-MEL; Lymph node homing receptor; LYAM1; LECAM1; LEU8; LAM-1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SELL |
|---|---|
| Gene Full Name | selectin L |
| Background | This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009] |
| Function | Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. [UniProt] |
| Highlight | Related products: CD62L antibodies; CD62L ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
-
ARG81782 Rat CD62L / L-Selectin ELISA Kit standard curve image
ARG81782 Rat CD62L / L-Selectin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81782 Rat sCD62L / sL-Selectin ELISA Kit User's manual |
 Download
|
