ARG82287
Porcine FGF basic ELISA Kit
Porcine FGF basic ELISA Kit for ELISA and Pig
Overview
| Product Description | ARG82287 Porcine FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Porcine FGF basic in serum and plasma. |
|---|---|
| Tested Reactivity | Pig |
| Tested Application | ELISA |
| Specificity | Not react with following recombinant porcine proteins: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HSP27, HGF, IL1 beta, IL1 RA, IL2, IL4, IL5, sFGF BASICR, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, MMP9, IL2R, PDGF, serpin E1, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TLR9, TNF alpha, TNF RI, TNF RII, VEGF and VEGF R1. |
| Target Name | FGF basic |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| Alternate Names | FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | FGF2 |
|---|---|
| Gene Full Name | fibroblast growth factor 2 (basic) |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| Function | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| Cellular Localization | Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. [UniProt] |
| Highlight | Related products: FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82287 Porcine FGF basic ELISA Kit standard curve image
ARG82287 Porcine FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82287 Porcine FGF basic ELISA Kit User manual |
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