ARG30213
Phospho p53 Antibody Duo (Total, pS15)
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG20150 | anti-p53 phospho (Ser15) antibody | Rabbit pAb | Hu, Ms, Rat | IHC, IP, WB | 25 μg |
| ARG51081 | anti-p53 antibody | Rabbit pAb | Hu | ICC/IF, IHC-P, WB | 50 μl |
Overview
| Product Description | P53, also known as tumor antigen p53, acts as a tumor suppressor in many tumor types to induce growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Upon gamma-irradiation and DNA damage, p53 is phosphorylated at Ser15 residue and this increases its ability to recruit CBP/p300 which in turns increase the overall acetylation of C-terminus of p53 protein. Acetylation of C-terminal domain can dramatically increase site-specific DNA binding ability pf p53 protein on both short oligonucleotides and long DNA fragments. Zhao et al. 2008. Cell cycle 7(19): 3048-55 Lambert et al. 1998. J Biol Chem 273(49): 33048 Luo et al. 2004. PNAS 101(8): 2259 Related news: Immune signaling protein TLR4 has opposing roles in breast cancer development Therapeutic strategies against PDAC |
|---|---|
| Target Name | p53 |
| Alternate Names | Phospho p53 antibody; Phospho tumor protein p53 antibody; p53 phospho (Ser15) antibody; p53 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Phospho tumor protein p53 Antibody Duo (Total, pS15) |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody |
Images (4) Click the Picture to Zoom In
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ARG20150 anti-P53 phospho (Ser15) antibody WB image
Western Blot: 1. Untreated HeLa cell 2. UV-traeted HeLa cell stained with anti-p53 (phospho Ser15) antibody (ARG20150).
The antibody detects ~53 kDa Ser15 phosphorylated p53. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites. -
ARG51081 anti-p53 antibody WB image
Western Blot: extracts from MDA and JK cells stained with anti-p53 antibody ARG51081.
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ARG51081 anti-p53 antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-p53 antibody ARG51081 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51081 anti-p53 antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-p53 antibody ARG51081.
