ARG30033
Phospho ZAP70 Antibody Duo (Total, pY319)
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51657 | anti-ZAP70 phospho (Tyr319) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, WB | 50 μl |
| ARG65322 | anti-ZAP70 antibody | Goat pAb | Hu | IP, WB | 50 μg |
Overview
| Product Description | ZAP70, Zeta-chain-associated protein kinase 70 (70kDa), is a tyrosine kinase protein normally expressed near the surface membrane of T cells and natural killer cells and plays a critical role in the events involved in initiating T-cell responses by the antigen receptor. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation. |
|---|---|
| Target Name | ZAP70 |
| Alternate Names | Phospho ZAP70 antibody; Phospho Zeta chain of T cell receptor associated protein kinase 70 antibody; ZAP70 phospho (Tyr319) antibody; ZAP70 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Phospho Zeta chain of T cell receptor associated protein kinase 70 (ZAP70) Antibody Duo (Total, pY319) |
|---|---|
| Research Area | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody |
Images (5) Click the Picture to Zoom In
-
ARG65322 anti-ZAP70 antibody WB image
Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65322 anti-ZAP70 antibody at 1:500 dilution.
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody WB image
Western Blot: extracts from Jurkat cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody WB image
Western Blot: extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
-
ARG65322 anti-ZAP70 antibody WB image
Western Blot: Jurkat lysate (35 µg protein in RIPA buffer) stained with ARG65322 anti-Zap70 antibody at 0.3 µg/ml dilution.
-
ARG51657 anti-Zap-70 phospho (Tyr319) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-Zap-70 (phospho Tyr319) antibody ARG51657.
