ARG65322
anti-ZAP70 antibody
anti-ZAP70 antibody for Immunoprecipitation,Western blot and Human
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody
1
Overview
| Product Description | Goat Polyclonal antibody recognizes ZAP70 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IP, WB |
| Specificity | This antibody is expected to recognize isoform 1 (NP_001070.2) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ZAP70 |
| Antigen Species | Human |
| Immunogen | Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2. |
| Conjugation | Un-conjugated |
| Alternate Names | STD; SRK; STCD; 70 kDa zeta-chain associated protein; Tyrosine-protein kinase ZAP-70; TZK; Syk-related tyrosine kinase; ZAP-70; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ZAP70 |
| Gene Full Name | zeta chain of T cell receptor associated protein kinase 70 |
| Background | This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30033 Phospho ZAP70 Antibody Duo (Total, pY319) ARG30049 SyK / Zap70 Antibody Duo Related products: ZAP70 antibodies; ZAP70 Duos / Panels; Anti-Goat IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody; SyK / Zap70 Pathway antibody |
| Calculated MW | 70 kDa |
| PTM | Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B. |
Images (1) Click the Picture to Zoom In
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ARG65322 anti-ZAP70 antibody WB image
Western blot: 30 µg of Jurkat and Molt4 cell lysates stained with ARG65322 anti-ZAP70 antibody at 1:500 dilution.
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Review for anti-ZAP70 antibody
Application：WB
Sample：Jurkat and Molt4
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
