ARG30353
Phospho IKB alpha Antibody Duo (Total, pS32 / 36)
Publication1
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51651 | anti-IKB alpha phospho (Ser32 / Ser36) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG40630 | anti-IKB alpha antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, IP, WB | 50 μl |
Overview
| Product Description | IκB-α inhibits the activity of dimeric NF-kappa-B / REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, triggers IκB-α phosphorylated at Ser32 / 36 and promotes IκB-α ubiquitination and degradation, enabling the dimeric NFkB to translocate to the nucleus and activate transcription._x000D_ _x000D_ ARG30053 IκB-α phospho Duos (Total, pS32 / 36), includes an antibody reacts total IκB-α protein and an antibody recognizes IκB-α only when Ser32 / 36 of IκB-α is phosphorylated. This antibody Duos could be used in evaluation of total or Ser32 / 36 phosphorylated IκB-α protein in IκB-α / NFkB signaling and IκB-α / NFkB regulation studies. |
|---|---|
| Target Name | Phospho IKB alpha |
| Alternate Names | Phospho nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IKB alpha) Antibody Duo (Total, pS32/36) |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | |Phospho IKB alpha Antibody Duo Total, pS32/36|anti IkB alpha antibody2E10 B11 F6, N terminal|2E10 B11 F6|NF kappa B inhibitor alpha|I kappa B alpha|IkB alpha|IkappaBalpha|Major histocompatibility complex enhancer binding protein MAD3|2E10 B11 F6|IKB alpha|IKB alpha|anti IKB alpha phospho Ser32/Ser36 antibody|NFKBIA|nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha|IKBA|MAD3|NFKBI|NF kappa B inhibitor alpha|I kappa B alpha|IkB alpha|IkappaBalpha|Major histocompatibility complex enhancer binding protein MAD3|IKBA|MAD3|NFKBI|IKB alpha|IKB alpha| |
|---|---|
| Highlight | Related Product: anti-IKB alpha antibody; |
Images (7) Click the Picture to Zoom In
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ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image
Western blot: Rat cardiac stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody at dilution.
From Yongjun Mao et al. Biomed Pharmacother. (2020), doi: 10.1016/j.biopha.2019.109280, Fig. 6D.
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ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with TNF stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
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ARG40630 anti-IKB alpha antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse stomach tissue stained with ARG40630 anti-IKB alpha antibody.
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ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody (left) or the same antibody preincubated with blocking peptide (right).
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ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
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ARG40630 anti-IKB alpha antibody WB image
Western blot: HeLa cell lysate stained with ARG40630 anti-IKB alpha antibody.
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ARG40630 anti-IKB alpha antibody ICC/IF image
Immunofluorescence: Hela stained with ARG40630 anti-IKB alpha antibody.
Specific References
iRhom2 deficiency reduces sepsis-induced mortality associated with the attenuation of lung macrophages in mice
ARG51651: IHC-P / Mouse
