ARG30353

Phospho IKB alpha Antibody Duo (Total, pS32 / 36)

Datasheet

Publication1

Ordering Information

Package

Price (USD)

1 pair

469.00

Add to cart

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG51651	anti-IKB alpha phospho (Ser32 / Ser36) antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, WB	50 μl
ARG40630	anti-IKB alpha antibody	Rabbit pAb	Hu, Ms, Rat	ICC/IF, IHC-P, IP, WB	50 μl

Overview

Product Description	IκB-α inhibits the activity of dimeric NF-kappa-B / REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, triggers IκB-α phosphorylated at Ser32 / 36 and promotes IκB-α ubiquitination and degradation, enabling the dimeric NFkB to translocate to the nucleus and activate transcription. ARG30053 IκB-α phospho Duos (Total, pS32 / 36), includes an antibody reacts total IκB-α protein and an antibody recognizes IκB-α only when Ser32 / 36 of IκB-α is phosphorylated. This antibody Duos could be used in evaluation of total or Ser32 / 36 phosphorylated IκB-α protein in IκB-α / NFkB signaling and IκB-α / NFkB regulation studies.
Target Name	Phospho IKB alpha
Alternate Names	Phospho nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IKB alpha) Antibody Duo (Total, pS32/36)

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Background	\|Phospho IKB alpha Antibody Duo Total, pS32/36\|anti IkB alpha antibody2E10 B11 F6, N terminal\|2E10 B11 F6\|NF kappa B inhibitor alpha\|I kappa B alpha\|IkB alpha\|IkappaBalpha\|Major histocompatibility complex enhancer binding protein MAD3\|2E10 B11 F6\|IKB alpha\|IKB alpha\|anti IKB alpha phospho Ser32/Ser36 antibody\|NFKBIA\|nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha\|IKBA\|MAD3\|NFKBI\|NF kappa B inhibitor alpha\|I kappa B alpha\|IkB alpha\|IkappaBalpha\|Major histocompatibility complex enhancer binding protein MAD3\|IKBA\|MAD3\|NFKBI\|IKB alpha\|IKB alpha\|

Background

Images (7) Click the Picture to Zoom In

ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

Western blot: Rat cardiac stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody at dilution.

From Yongjun Mao et al. Biomed Pharmacother. (2020), doi: 10.1016/j.biopha.2019.109280, Fig. 6D.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image

Western blot: Extracts from HeLa cells untreated or treated with TNF stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
ARG40630 anti-IKB alpha antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Mouse stomach tissue stained with ARG40630 anti-IKB alpha antibody.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody (left) or the same antibody preincubated with blocking peptide (right).
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody ICC/IF image

Immunofluorescence: methanol-fixed HeLa cells stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
ARG40630 anti-IKB alpha antibody WB image

Western blot: HeLa cell lysate stained with ARG40630 anti-IKB alpha antibody.
ARG40630 anti-IKB alpha antibody ICC/IF image

Immunofluorescence: Hela stained with ARG40630 anti-IKB alpha antibody.