ARG30227
Phospho ATF2 Antibody Panel (Total, pT69, pT71)
Gene Regulation antibody; Immune System antibody
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51535 | anti-ATF2 phospho (Thr71 or 53) antibody | Rabbit pAb | Hu, Ms, Rat | IHC-P, WB | 50 μl |
| ARG51534 | anti-ATF2 phospho (Thr69 or 51) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG63122 | anti-ATF2 antibody | Goat pAb | Hu | WB | 50 μg |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
| ARG65352 | Donkey anti-Goat IgG antibody (HRP) | Donkey pAb | Goat | ELISA, IHC, WB | 50 μl |
Overview
| Product Description | The activating transcription factor (ATF) and cAMP-response-element binding (CREB) families of transcription factors comprise 16 members of the activator protein 1 (AP1) transcription factor superfamily. ATF and CREB proteins can homodimerize or heterodimerize with members of the Jun, Fos or Maf transcription factor families to form complexes that regulate diverse cellular functions, such as stress responses, embryonic development, disease development and cell death. ATF2 requires phosphorylation by Jun N-terminal kinase (JNK), p38 (MAPK14), or extracellular-signal-regulated kinase 1 (ERK1) at Thr69 and Thr71 residues in order to be transcriptionally active. Lau and Ronai. 2012. J Cell Sci 125:2815 Morton et al. 2004. FEBS Lett 572:177-83 Waas et al. 2001. J Biol Chem 276(8):5676 |
|---|---|
| Target Name | ATF2 |
| Alternate Names | Phospho ATF2 antibody; Phospho Activating transcription factor 2 antibody; ATF2 phospho (Thr69 or 51) antibody; ATF2 phospho (Thr71 or 53) antibody; ATF2 antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Phospho Activating transcription factor 2 (ATF2) Antibody Panel (Total, pT69, pT71) |
|---|---|
| Research Area | Gene Regulation antibody; Immune System antibody |
Images (7) Click the Picture to Zoom In
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ARG51534 anti-ATF2 phospho (Thr69 or 51) antibody WB image
Western Blot: extracts from 3T3 cells untreated or treated with Anisomycin stained with anti-ATF2 (phospho Thr69 or 51) antibody ARG51534.
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ARG51534 anti-ATF2 phospho (Thr69 or 51) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-ATF2 (phospho Thr69 or 51) antibody ARG51534 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51534 anti-ATF2 phospho (Thr69 or 51) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-ATF2 (phospho Thr69 or 51) antibody ARG51534.
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ARG51535 anti-ATF2 phospho (Thr71 or 53) antibody WB image
Western Blot: extracts from HUVEC cells untreated or treated with Anisomycin stained with anti-ATF2 (phospho Thr71 or 53) antibody ARG51535.
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ARG51535 anti-ATF2 phospho (Thr71 or 53) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-ATF2 (phospho Thr71 or 53) antibody ARG51535 (left) or the same antibody preincubated with blocking peptide (right).
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ARG63122 anti-ATF2 antibody WB image
Western Blot: Human Placenta lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63122 anti-ATF2 antibody at 0.5 µg/ml dilution.
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ARG63122 anti-ATF2 antibody WB image
Western Blot: HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35 µg protein in RIPA buffer) stained with ARG63122 anti-ATF2 antibody at 1 µg/ml dilution.
