ARG82529
Mouse PODXL ELISA Kit
Mouse PODXL ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82529-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82529-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82529-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82529-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82529-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82529-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82529-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82529-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82529-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82529-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82529-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82529 Mouse PODXL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse PODXL in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | PODXL |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1% Inter-Assay CV: 6.9% |
| Alternate Names | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PODXL |
| Gene Full Name | podocalyxin-like |
| Background | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| Cellular Localization | Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: PODXL antibodies; PODXL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82529 Mouse PODXL ELISA Kit standard curve image
ARG82529 Mouse PODXL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82529 Mouse PODXL ELISA Kit User manual |
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