ARG81668
Mouse OPN / Osteopontin ELISA Kit
Mouse OPN / Osteopontin ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81668-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81668-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81668-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81668-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81668-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81668-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81668-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81668-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81668-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81668-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81668-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81668 Mouse OPN / Osteopontin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse OPN / Osteopontin in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | OPN / Osteopontin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.1%; Inter-Assay CV: 7.0% |
| Alternate Names | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPP1 |
| Gene Full Name | secreted phosphoprotein 1 |
| Background | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| Highlight | Related products: OPN antibodies; OPN ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81668 Mouse OPN / Osteopontin ELISA Kit standard curve image
ARG81668 Mouse OPN / Osteopontin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81668 Mouse Osteopontin ELISA Kit User manual |
 Download
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