ARG81811
Mouse Granzyme B ELISA Kit
Mouse Granzyme B ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81811-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81811-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81811-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81811-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81811-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81811-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81811-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81811-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81811-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81811-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81811-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81811 Mouse Granzyme B ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Granzyme B in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | Granzyme B |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2% |
| Alternate Names | EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GZMB |
| Gene Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Background | Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| Function | Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt] |
| Highlight | Related products: Granzyme B antibodies; Granzyme B ELISA Kits; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA Anti-SerpinB9 therapy, a new strategy for cancer therapy New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81811 Mouse Granzyme B ELISA Kit standard curve image
ARG81811 Mouse Granzyme B ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81811 Mouse Granzyme B ELISA Kit User's manual |
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