ARG82328
Mouse CD152 / CTLA4 ELISA Kit
Mouse CD152 / CTLA4 ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82328-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82328-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82328-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82328-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82328-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82328-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82328-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82328-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82328-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82328-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82328-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82328 Mouse CD152 / CTLA4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD152 / CTLA4 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | CD152 / CTLA4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| Alternate Names | GRD4; CTLA-4; CELIAC3; CD; Cytotoxic T-lymphocyte-associated antigen 4; CD152; GSE; CD antigen CD152; Cytotoxic T-lymphocyte protein 4; ALPS5; IDDM12 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CTLA4 |
| Gene Full Name | cytotoxic T-lymphocyte-associated protein 4 |
| Background | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. [provided by RefSeq, Jul 2008] |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;. [UniProt] |
| Highlight | Related products: CD152 antibodies; CD152 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG82328 Mouse CD152 / CTLA4 ELISA Kit standard curve image
ARG82328 Mouse CD152 / CTLA4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82328 Mouse CD152 / CTLA4 ELISA Kit User manual |
 Download
|
