ARG83169

Monkey MIF ELISA Kit

Monkey MIF ELISA Kit for ELISA and Monkey

Overview

Product Description ARG83169 Monkey MIF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Monkey MIF in Serum, Plasma, Monkey Milk and Cell culture supernatants.
Tested Reactivity Mk
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name MIF
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 25 pg/ml
Detection Range 156 pg/ml - 10,000 pg/ml
Sample Type Serum, Plasma, Monkey Milk and Cell culture supernatants
Precision Intra-Assay CV: 5.1%
Inter-Assay CV: 6.4%
Alternate Names Phenylpyruvate tautomerase; L-dopachrome tautomerase; GLIF; L-dopachrome isomerase; MMIF; Macrophage migration inhibitory factor; MIF; GIF; EC 5.3.2.1; Glycosylation-inhibiting factor; EC 5.3.3.12

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 574298 Monkey MIF

Swiss-port # Q6DN04 Monkey Macrophage migration inhibitory factor

Gene Symbol MIF
Gene Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Background This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
Function Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. [UniProt]
Highlight Related products:
MIF antibodies; MIF ELISA Kits; MIF Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Images (1) Click the Picture to Zoom In

  • ARG83169 Monkey MIF ELISA Kit standard curve image

    ARG83169 Monkey MIF ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG83169 Monkey MIF ELISA Kit User manual download icon Download