ARG82827
Human alpha Fetoprotein ELISA Kit
Human alpha Fetoprotein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82827-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82827-002 | Standard | 2 X 5 ng/vial | 4°C |
| ARG82827-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82827-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82827-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82827-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82827-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82827-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82827-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82827-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82827-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82827 Human alpha Fetoprotein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Fetoprotein in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | alpha Fetoprotein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 39 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.6% Inter-Assay CV: 6.9% |
| Alternate Names | Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AFP |
| Gene Full Name | alpha-fetoprotein |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019] |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82827 Human alpha Fetoprotein ELISA Kit standard curve image
ARG82827 Human alpha Fetoprotein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82827 Human alpha Fetoprotein ELISA Kit User manual |
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