ARG82827

Human alpha Fetoprotein ELISA Kit

Human alpha Fetoprotein ELISA Kit for ELISA and Human

Component

Cat No Component Name Package Temp
ARG82827-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82827-002 Standard 2 X 5 ng/vial 4°C
ARG82827-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82827-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82827-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82827-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82827-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82827-008 25X Wash buffer 20 ml 4°C
ARG82827-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82827-010 STOP solution 10 ml (Ready to use) 4°C
ARG82827-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82827 Human alpha Fetoprotein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Fetoprotein in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Target Name alpha Fetoprotein
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 39 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 78 - 5000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.6%
Inter-Assay CV: 6.9%
Alternate Names Alpha-fetoprotein; HPAFP; FETA; Alpha-fetoglobulin; AFPD; Alpha-1-fetoprotein

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 174 Human AFP

Swiss-port # P02771 Human Alpha-fetoprotein

Gene Symbol AFP
Gene Full Name alpha-fetoprotein
Background This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Oct 2019]
Function Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. [UniProt]
Cellular Localization Secreted. [UniProt]
PTM Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.

Sulfated. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82827 Human alpha Fetoprotein ELISA Kit standard curve image

    ARG82827 Human alpha Fetoprotein ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82827 Human alpha Fetoprotein ELISA Kit User manual download icon Download