ARG81468
Human SERPINA4 / Kallistatin ELISA Kit
Human SERPINA4 / Kallistatin ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81468-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81468-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81468-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81468-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81468-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81468-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81468-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81468-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81468-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81468-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81468-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81468 Human SERPINA4 / Kallistatin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SERPINA4 / Kallistatin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | SERPINA4 / Kallistatin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 5.9% |
| Alternate Names | KST; PI-4; kallistatin; Peptidase inhibitor 4; KLST; KAL; Serpin A4; Kallikrein inhibitor; PI4; Kallistatin |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINA4 |
| Gene Full Name | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 |
| Function | Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. [UniProt] |
| Highlight | Related products: Serpin antibodies; Serpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The N-terminus is blocked. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81468 Human SERPINA4 / Kallistatin ELISA Kit standard curve image
ARG81468 Human SERPINA4 / Kallistatin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81468 Human SERPINA4 / Kallistatin ELISA Kit User's manual |
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