ARG80142
Human PLGF ELISA Kit
Human PLGF ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80142-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80142-002 | Standard (Lyophilized) | 2 X 3 ng/vial | 4°C |
| ARG80142-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80142-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80142-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80142-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80142-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80142-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80142-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80142-010 | STOP solution | 12ml | 4°C |
| ARG80142-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG80142 Human PLGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLGF in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | No significant cross-reactivity or interference with Human EGF, PDGF-AA, PDGF-BB, VEGF121, VEGF165 and mouse VEGF165. |
| Target Name | PLGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 15 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 31.25 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | CV: <10% |
| Alternate Names | PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF |
Application Instructions
| Assay Time | 4 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PGF |
| Gene Full Name | placental growth factor |
| Background | This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] |
| Function | Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt] |
| Cellular Localization | Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. [UniProt] |
| Highlight | Related products: PLGF antibodies; PLGF ELISA Kits; Related news: The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit |
| PTM | N-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG80142 Human PLGF1 ELISA Kit standard curve image
ARG80142 Human PLGF1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80142 Human PLGF1 ELISA Kit User's manual |
 Download
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