ARG83437
Human Cytochrome C ELISA Kit
Human Cytochrome C ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83437 Human Cytochrome C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cytochrome C in Cell Lysates |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | Cytochrome C |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 40 pg/ml |
| Sample Type | Cell Lysates |
| Standard Range | 78 - 5000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| Alternate Names | CYC; HCS; Cytochrome c; THC4 |
Application Instructions
| Assay Time | 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CYCS |
| Gene Full Name | cytochrome c, somatic |
| Background | Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases. |
| Function | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt] |
| Cellular Localization | Mitochondrion |
| PTM | Acetylation, Phosphoprotein |
Images (1) Click the Picture to Zoom In
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ARG83437 Human Cytochrome C ELISA Kit standard curve image
ARG83437 Human Cytochrome C ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83437 Human Cytochrome C ELISA Kit User manual |
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