ARG81441
Human CD163 ELISA Kit
Human CD163 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81441-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81441-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81441-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81441-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81441-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81441-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81441-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81441-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81441-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81441-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81441-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81441 Human CD163 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CD163 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | CD163 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.78 ng/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 1.56 - 100 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.1% |
| Alternate Names | sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q86VB7 Human Scavenger receptor cysteine-rich type 1 protein M130 |
|---|---|
| Gene Symbol | CD163 |
| Gene Full Name | CD163 molecule |
| Background | CD163 protein is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] |
| Function | CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt] |
| Highlight | Related products: CD163 antibodies; CD163 ELISA Kits; CD163 Duos / Panels; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81441 Human CD163 ELISA Kit standard curve image
ARG81441 Human CD163 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81441 Human CD163 ELISA Kit User's manual |
 Download
|
