ARG81577
Human AGRP / Agouti-related protein ELISA Kit
Human AGRP / Agouti-related protein ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81577-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81577-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81577-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81577-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81577-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81577-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81577-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81577-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81577-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81577-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81577-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81577 Human AGRP / Agouti-related protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AGRP / Agouti-related protein in serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | AGRP / Agouti related protein |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 3.9 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate), urine and cell culture supernatants. |
| Standard Range | 7.8 - 500 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.2% Inter-Assay CV: 5.2% |
| Alternate Names | Agouti-related protein; AGRT; ART; ASIP2 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AGRP |
| Gene Full Name | agouti related neuropeptide |
| Background | This gene encodes an antagonist of the melanocortin-3 and melanocortin-4 receptor. It appears to regulate hypothalamic control of feeding behavior via melanocortin receptor and/or intracellular calcium regulation, and thus plays a role in weight homeostasis. Mutations in this gene have been associated with late on-set obesity. [provided by RefSeq, Dec 2009] |
| Function | Plays a role in weight homeostasis. Involved in the control of feeding behavior through the central melanocortin system. Acts as alpha melanocyte-stimulating hormone antagonist by inhibiting cAMP production mediated by stimulation of melanocortin receptors within the hypothalamus and adrenal gland. Has very low activity with MC5R (By similarity). Is an inverse agonist for MC3R and MC4R being able to suppress their constitutive activity. It promotes MC3R and MC4R endocytosis in an arrestin-dependent manner. [UniProt] |
| Highlight | Related products: AGRP antibodies; AGRP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81577 Human AGRP / Agouti-related protein ELISA Kit standard curve image
ARG81577 Human AGRP / Agouti-related protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81577 Human AGRP / Agouti-related protein ELISA Kit User manual |
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