ARG80959
Human ACE2 ELISA Kit
Human ACE2 ELISA Kit for ELISA and Human
Cell Biology and Cellular Response kit
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG80959-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80959-002 | Standard | 2 X 1 ng/vial | 4°C |
| ARG80959-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG80959-004 | Antibody conjugate concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG80959-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG80959-006 | HRP-Streptavidin concentrate (100X) | 1 vial (120 µl) | 4°C |
| ARG80959-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG80959-008 | 20X Wash buffer | 30 ml | 4°C |
| ARG80959-009 | TMB substrate | 12 ml (Ready to use) | 4°C (Protect from light) |
| ARG80959-010 | STOP solution | 12 ml (Ready to use) | 4°C |
| ARG80959-011 | Plate sealer | 3 strips | Room temperature |
Overview
| Product Description | ARG80959 Human ACE2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACE2 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ACE2 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 8 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 5.7% |
| Alternate Names | Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACE2 |
| Gene Full Name | angiotensin I converting enzyme 2 |
| Background | The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008] |
| Function | Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt] |
| Highlight | Related products: ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 ACE2, receptor of 2019-nCoV New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Cell Biology and Cellular Response kit |
| PTM | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. |
Images (1) Click the Picture to Zoom In
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ARG80959 Human ACE2 ELISA Kit standard curve image
ARG80959 Human ACE2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80959 Human ACE2 ELISA Kit User's manual |
 Download
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