ARG23705

Goat anti-Mouse IgG2a antibody (HRP), pre-adsorbed

Goat anti-Mouse IgG2a antibody (HRP), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Mouse

Overview

Product Description HRP-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
Tested Reactivity Ms
Species Does Not React With Hu
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG2a
Antigen Species Mouse
Immunogen IgG2a
Conjugation HRP

Application Instructions

Pre Adsorbed Mouse IgM, IgG1, IgG2b, IgG3 and IgA; Pooled human sera; Purified human paraproteins.
Application Suggestion
Tested Application Dilution
ELISA1:4000 - 1:8000
ELISPOTAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4) and 50% Glycerol.
Stabilizer 50% Glycerol
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (4) Click the Picture to Zoom In

  • ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue stained with ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] (left) and ARG22191 anti-MMP9 antibody [SB15c] (right), followed by ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed), DAB and hematoxylin.

  • ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed) WB image

    Western blot: Mouse thymocytes and HepG2 cell lysates stained with ARG22305 anti-Bcl XL antibody [2H12]. Secondary antibody: ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed).

  • ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed).

  • ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney cancer tissue stained with ARG21961 Mouse IgG2a Isotype Control antibody [HOPC-1] (left) and anti-MMP3 antibody (right) followed by ARG23705 Goat anti-Mouse IgG2a antibody (HRP) (pre-adsorbed), DAB and hematoxylin.