ARG30314

Chondrogenesis Marker Antibody Panel

Datasheet

Publication2

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1 kit

869.00

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Datasheet
Publication (2)

Component

Cat No	Component Name	Host clonality	Reactivity	Application	Package
ARG57983	anti-CDKN2A / p16INK4a antibody	Rabbit pAb	Hu	FACS, ICC/IF, IHC-P, IP, WB	50 μl
ARG63341	anti-FOXC1 antibody	Goat pAb	Hu	FACS, ICC/IF, IHC-P	50 μg
ARG63332	anti-FOXC2 antibody	Goat pAb	Hu	FACS, IHC-P, WB	50 μg
ARG62852	anti-CD44 antibody [MEM-263]	Mouse mAb	Hu, Dog, Pig	FACS, IHC-P, IP, WB	50 μg
ARG65350	Goat anti-Mouse IgG antibody (HRP)	Goat pAb	Ms	ELISA, IHC-P, WB	50 μl
ARG65351	Goat anti-Rabbit IgG antibody (HRP)	Goat pAb	Rb	ELISA, IHC-P, WB	50 μl
ARG65352	Donkey anti-Goat IgG antibody (HRP)	Donkey pAb	Goat	ELISA, IHC, WB	50 μl

Overview

Product Description	Chondrogenesis is a process involving Mesenchymal Stem Cells (MSCc) condensation leading to chondroprogenitor cell differentiation. The main function of chondrogenensis is to generate cartilage during fetal development, and gradually replacing cartilage with bone at adulthood. Transcription factor SOX9, FOXC1 and FOXC2 are critical for driving chondrocyte differentiation. Hyaluronan receptor CD44 plays important role in cell-matrix interaction during chondrogenesis and matrix assembly. Hardinghan et al (2006). J Anat 209(4): 469-80 Wilm et al (2004). Dev Biol 271:176-189 Knudson CB (2003). Birth Defects Res C Embryo Today 69(2):174-96
Target Name	Chondrogenesis Marker
Alternate Names	Chondrogenesis Marker antibody; CDKN2A / p16INK4a antibody; CD44 antibody; FOXC2 antibody; FOXC1 antibody

Properties

Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Images (11) Click the Picture to Zoom In

ARG63341 anti-FOXC1 antibody ICC/IF image

Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG63341 anti-FOXC1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63341 anti-FOXC1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63341 anti-FOXC1 antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG57983 anti-CDKN2A / p16INK4a antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human gastric stained with ARG57983 anti-CDKN2A / p16INK4a antibody.
ARG63332 anti-FOXC2 antibody WB image

Western blot: 35 µg of HEK293 nucleus (A) and Human pancreas (B, negative control) lysates (in RIPA buffer) stained with ARG63332 anti-FOXC2 antibody at 2 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
ARG63341 anti-FOXC1 antibody IHC-P image

Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63341 anti-FOXC1 antibody at 4 µg/ml dilution followed by HRP-staining.
ARG62852 anti-CD44 antibody [MEM-263] WB image

Western blot: Isolated peripheral blood lymphocytes (PBL) of various species. Human PBL, Dog PBL and Pig PBL lysates stained with ARG62852 anti-CD44 antibody [MEM-263], in non-reducing conditions.
ARG57983 anti-CDKN2A / p16INK4a antibody WB image

Western blot: 293T cell lysate stained with ARG57983 anti-CDKN2A / p16INK4a antibody.
ARG63332 anti-FOXC2 antibody FACS image

Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63332 anti-FOXC2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
ARG63341 anti-FOXC1 antibody FACS image

Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63341 anti-FOXC1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
ARG63341 anti-FOXC1 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63341 anti-FOXC1 antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG62852 anti-CD44 antibody [MEM-263] WB image

Western blot: MOLT-4 and HeLa cell lysates stained with ARG62852 anti-CD44 antibody [MEM-263], in non-reducing conditions.