ARG30304
Astrocyte Maturation / Muller Cell Marker Antibody Duo (GFAP, Vimentin)
Publication16
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG10122 | anti-GFAP antibody [GF5] | Mouse mAb | Hu, Ms, Rat | ELISA, ICC/IF, IHC-Fr, WB | 50 μg |
| ARG66302 | anti-Vimentin antibody [SQab1859] | Mouse mAb | Hu, Ms, Rat, Chk, Dog, Goat, Hm, Mk, Pig, Xenopus, Zfsh | FACS, ICC/IF, IHC-Fr, IHC-P, WB | 50 μg |
Overview
| Product Description | Glial fibrillary acidic protein (GFAP) is the major intermediate filament protein in mature astrocytes, a main type of glial cells in the central nervous system (CNS). GFAP is a fairly astrocyte marker. Vimentin is a type III intermediate filament protein present in many cell types of mesodermal origin as well as some ectodermal cells, including neural stem cells. Vimentin is expressed in transient cell type radial glia during development. After maturation, most radial glia disappear and transform into stellate astrocytes, although radial glial cells remain in the retina (Müller cells). arigo’s ARG30088 Astrocyte Maturation / Muller Cell Marker Antibody Duo (GFAP, Vimentin) comprises Vimentin and GFAP antibodies for identifying immature and mature astrocytes respectively. It is also excellent for labeling Müller cells in the retina. Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
|---|---|
| Target Name | Astrocyte Maturation / Muller Cell Marker |
| Alternate Names | Astrocyte Maturation / Muller Cell Marker antibody; GFAP antibody; Vimentin antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (11) Click the Picture to Zoom In
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ARG10122 anti-GFAP antibody [GF5] ICC/IF image
Immunofluorescence: Rat astrocyte primary cell stained with ARG10122 anti-GFAP antibody [GF5] (green) at 1:200 dilution.
Cell nuclei was stained with DAPI (blue). -
ARG66302 anti-Vimentin antibody [SQab1859] IHC-P image
Immunohistochemistry: Mouse xenograft tumor stained with ARG66302 anti-Vimentin antibody [SQab1859].
From Jianxia Wei et al. Cancer Sci. (2023), doi: 10.1111/cas.15998, Fig. 6E.
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ARG10122 anti-GFAP antibody [GF5] IHC-Fr image
Immunohistochemistry: Rat ventral horn of spinal cord stained with ARG10122 anti-GFAP antibody [GF5] at 1: 500 dilution.
From Chin-An Chen et al. Int J Med Sci. (2021), doi: 10.7150/ijms.65976, Fig. 4A.
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ARG66302 anti-Vimentin antibody [SQab1859] WB image
Western blot: 20 µg of 22RV1 and DU145 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.
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ARG66302 anti-Vimentin antibody [SQab1859] WB image
Western blot: 20 µg of COLO205 and SW620 cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:2000 dilution and ARG65680 anti-GAPDH antibody at 1:10000 dilution.
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ARG10122 anti-GFAP antibody [GF5] WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.
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ARG66302 anti-Vimentin antibody [SQab1859] IHC-Fr image
Immunohistochemistry: Frozen section of swine colon stained with ARG66302 anti-Vimentin antibody [SQab1859] (green) at 1:200 dilution. DAPI (blue) staining for cell nuclei.
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ARG10122 anti-GFAP antibody [GF5] WB image
Western blot: 30 µg of Rat brain lysate stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.
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ARG66302 anti-Vimentin antibody [SQab1859] WB image
Western blot: 20 µg of 293T cell lysate stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.
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ARG66302 anti-Vimentin antibody [SQab1859] WB image
Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG66302 anti-Vimentin antibody [SQab1859] at 1:1000 dilution.
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ARG66302 anti-Vimentin antibody [SQab1859] IHC-P image
Immunohistochemistry: paraffin section of Human colon stained with ARG66302 anti-Vimentin antibody [SQab1859].
Specific References
Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment
ARG10122: IHC-Fr / Mouse
Norepinephrine-Activated p38 MAPK Pathway Mediates Stress-Induced Cytotoxic Edema of Basolateral Amygdala Astrocytes
ARG10122: WB, IHC-P, ICC/IF / Rat
CircBRD7 attenuates tumor growth and metastasis in nasopharyngeal carcinoma via epigenetic activation of its host gene
ARG66302: IHC-P, WB / Mouse
Effects of Chronic Restraint Stress on Apoptosis of Amygdala Cells in Rats
ARG10122: IHC-P / Rat
SCM-198 Alleviates Endometriosis by Suppressing Estrogen-ERα mediated Differentiation and Function of CD4+CD25+ Regulatory T Cells
ARG10122: WB / Mouse
Secreted Neutrophil Gelatinase-Associated Lipocalin Shows Stronger Ability to Inhibit Cyst Enlargement of ADPKD Cells Compared with Nonsecreted Form
ARG10122: WB / Mouse
SCM-198 Prevents Endometriosis By Reversing Low Autophagy Levels Of Endometriotic Stromal Cells Via Inhibiting TNF-Α-Aromatase-Estrogen-Erα Pathway And Promoting PR Expression
ARG10122: WB / Human
Hyperbaric Oxygen Therapy Attenuates Burn-Induced Denervated Muscle Atrophy
ARG10122: IHC-Fr / Rat
RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy
ARG10122: IHC-Fr / Rat
iTRAQ-Based Proteomics Reveals Gu-Ben-Fang-Xiao Decoction Alleviates Airway Remodeling via Reducing Extracellular Matrix Deposition in a Murine Model of Chronic Remission Asthma.
ARG66302: WB / Mouse
Human umbilical cord mesenchymal stem cell-derived exosomal miR-27b attenuates subretinal fibrosis via suppressing epithelial-mesenchymal transition by targeting HOXC6.
ARG66302: WB, ICC/IF / Human
SCM-198 ameliorates endometrial inflammation via suppressing the LPS-JNK-cJUN/cFOS-TLR4-NF-κB pathway
ARG10122: WB / Human
P4HA1 as an unfavorable prognostic marker promotes cell migration and invasion of glioblastoma via inducing EMT process under hypoxia microenvironment.
ARG66302: WB / Human
BRD7 suppresses invasion and metastasis in breast cancer by negatively regulating YB1-induced epithelial-mesenchymal transition.
ARG66302: WB, IHC-P / Human
Mechanisms of bupivacaine inhibition of glutamate-induced intracellular calcium elevation in primary cultured rat hippocampal astrocytes.
ARG10122: ICC/IF / Rat
Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain.
ARG10122: WB, IF, IHC-Fr / Rat
