ARG10122
anti-GFAP antibody [GF5]
anti-GFAP antibody [GF5] for ELISA,ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Publication8
Overview
| Product Description | Mouse Monoclonal antibody [GF5] recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC-Fr, WB |
| Specificity | This antibody is specific for Human GFAP. There is no cross-reactivity with other neurospecific proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GF5 |
| Isotype | IgG2b |
| Target Name | GFAP |
| Antigen Species | Human |
| Immunogen | 43-45 kD band corresponding to GFAP in immunoblotting of extract from human brain and spinal cord. |
| Conjugation | Un-conjugated |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 1.0-2.0 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFAP |
| Gene Full Name | glial fibrillary acidic protein |
| Background | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30304 Astrocyte Maturation / Muller Cell Marker Antibody Duo (GFAP, Vimentin) ARG30315 Brain Injury IHC Marker Antibody Duo (GFAP, MMP9) Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Microglial help TAM-ing inflammation in the brain Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| Calculated MW | 50 kDa |
| PTM | Phosphorylated by PKN1. |
Images (4) Click the Picture to Zoom In
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ARG10122 anti-GFAP antibody [GF5] ICC/IF image
Immunofluorescence: Rat astrocyte primary cell stained with ARG10122 anti-GFAP antibody [GF5] (green) at 1:200 dilution.
Cell nuclei was stained with DAPI (blue). -
ARG10122 anti-GFAP antibody [GF5] IHC-Fr image
Immunohistochemistry: Rat ventral horn of spinal cord stained with ARG10122 anti-GFAP antibody [GF5] at 1: 500 dilution.
From Chin-An Chen et al. Int J Med Sci. (2021), doi: 10.7150/ijms.65976, Fig. 4A.
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ARG10122 anti-GFAP antibody [GF5] WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.
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ARG10122 anti-GFAP antibody [GF5] WB image
Western blot: 30 µg of Rat brain lysate stained with ARG10122 anti-GFAP antibody [GF5] at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-GFAP antibody [GF5]
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-GFAP antibody [GF5]
Application：IF/ICC
Sample：Rat astrocyte primary cell
Fixation Buffer：PFA
Primary Antibody Dilution Factor：1:200
Specific References
Norepinephrine-Activated p38 MAPK Pathway Mediates Stress-Induced Cytotoxic Edema of Basolateral Amygdala Astrocytes
WB, IHC-P, ICC/IF / Rat
Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment
IHC-Fr / Mouse
Restraint stress promotes lung cancer xenograft growth via the IL2-Ras-Erk pathway
WB / Mouse
Effects of Chronic Restraint Stress on Apoptosis of Amygdala Cells in Rats
IHC-P / Rat
Hyperbaric Oxygen Therapy Attenuates Burn-Induced Denervated Muscle Atrophy
IHC-Fr / Rat
RIP3/MLKL pathway-regulated necroptosis: A new mechanism of paclitaxel-induced peripheral neuropathy
IHC-Fr / Rat
Mechanisms of bupivacaine inhibition of glutamate-induced intracellular calcium elevation in primary cultured rat hippocampal astrocytes.
ICC/IF / Rat
Annexin-1 Mimetic Peptide Ac2-26 Suppresses Inflammatory Mediators in LPS-Induced Astrocytes and Ameliorates Pain Hypersensitivity in a Rat Model of Inflammatory Pain.
WB, IF, IHC-Fr / Rat
