tdTomato, mRuby, and mCherry antibodies for triple staining

tdTomato, mRuby and mCherry are red fluorescent proteins widely used as a reporter in gene expression and protein localization studies. Although these fluorescent proteins are powerful tools to tracking gene expression or protein localization in live cells, it is difficult to visualize their fluorescence in fixed tissue sections due to the protein denaturation.

arigo offers quality tdTomato, mRuby and mCherry antibodies raised from goat. These antibodies are the best solution to visualize tdTomato, mRuby and mCherry in tissue sections as well as to make triple staining available when co-staining with rabbit and mouse antibodies.

 

Benefits
● Goat pAbs (Recommended 2o Ab: ARG65645) ● Good for ICC/IF, IHC-Fr, IHC-P, and WB ● Excellent for visualizing tdTomato/mCherry in tissue sections ● Ideal for triple immunofluorescence staining	tdTomato antibody ARG55724) mRuby antibody (ARG44152) mCherry antibody (ARG55723)

 

 

 

▶  tdTomato antibody (ARG55724)

Huang et al., (2024) Nat Commun. 15(1):9337. Ribeiro and Crossan (2023) Nucleic Acids Res. 51(10):4791-4813. Tang et al., (2023) Sci Adv. 9(34):eadh9570. Fan et al., (2023) Cell Mol Gastroenterol Hepatol. 15(2):511-531. (data as follows)

▶  mCherry antibody (ARG55723)

Huang et al., (2024) Dev Cell. 59(16):2101-2117.e8. (data on left) Wang et al., (2024) J Biol Chem. 300(8):107570. Song et al., (2023) NPJ Regen Med. 8(1):20. Wei et al., (2023) Cell Rep. 42(7):112660. (data on right) Cai et al., (2023) Cell Rep. 42(9):113028. Zhang et al., (2022) Cell Rep. 39(1):110596.

 

 

 

	GFP	RFP	mCherry
Chicken pAb	ARG10755		ARG10758
Goat pAb	ARG55727	ARG55744	ARG55723
Mouse mAb	ARG62343	ARG62344	ARG10759
Rabbit pAb	ARG62993		ARG10760

 

 

To facilitate the research of transcriptional regulation when using fluorescent promoter-reporters, arigo offers quality ELISA kits to simplify the quantification of RFP or GFP reporters in cell culture supernatants, cell lysates and tissue lysates.
 

RFP ELISA Kit (ARG81200) Good for quantifying tdTomato, mRuby, mCherry, mOrange, mStrawberry, mKate, mPlum, TagRFP, TurboRFP, and DsRed.

GFP ELISA Kit (ARG82206) Good for quantifying GFP, BFP, CFP, and YFP.

 

 

 

	S. aureus Cas9	S. pyogenes Cas9
Chicken pAb	ARG10681
Mouse mAb	ARG10705	ARG10706
Rabbit pAb	ARG41379	ARG10734