ARG55724
anti-tdTomato antibody
anti-tdTomato antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Other
Publication5
Overview
| Product Description | Goat Polyclonal antibody recognizes tdTomato |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This antibody recognizes tdTomato, mCherry, mApple and red fluorescent protein (DsRed). It does not cross-react to GFP. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | tdTomato |
| Immunogen | Purified full-length recombinant protein produced in E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Cherry fluorescent protein; DsRed; mCherry; red fluorescent protein; RFP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | tdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It's brightness and emission wavelength makes it ideal for live animal research. |
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Images (5) Click the Picture to Zoom In
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ARG55724 anti-tdTomato antibody IHC-Fr image
Immunohistochemistry: Mouse Tissue stained with ARG55724 anti-tdTomato antibody at 1:500 dilution.
From Maira Jalil et al. bioRxiv. (2023), doi: 10.1101/2023.12.16.571991, Fig. 4A, B.
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ARG55724 anti-tdTomato antibody IHC-P image
Immunohistochemistry: Mouse testis stained with ARG55724 anti-tdTomato antibody at 1:100 dilution.
From Jonathan Ribeiro et al. Nucleic Acids Res. (2023), doi: 10.1093/nar/gkad168, Fig. S5.
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ARG55724 anti-tdTomato antibody ICC/IF image
Immunofluorescence: N2a cells transfected with tdTomoto reporter plasmid and stained with ARG55724 anti-tdTomato antibody at 1:200 dilution. Cells were fixed with 4% of PFA.
Cell nuclei were stained with DAPI (blue). -
ARG55724 anti-tdTomato antibody IHC-P image
Immunohistochemistry:Paraffin-embedded Mouse Oviducts stained with ARG55724 anti-tdTomato antibody at 1:500 dilution (overnight at 4°C). Secondary antibody: Alexa Fluor 488-Goat anti-Goat (green) at 1:500 dilution (2 hours at RT).
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ARG55724 anti-tdTomato antibody WB image
Western blot: 50μg of mApple plasmid transfected HEK293 cell lysate stained with ARG55724 anti-tdTomato antibody at 1:2500 dilution.
Specific References
Availability of an inflammatory macrophage niche drives phenotypic and functional alterations in Kupffer cells
IHC-Fr / Mouse
GCNA is a histone binding protein required for spermatogonial stem cell maintenance
IHC-P / Mouse
Molecular Disambiguation of Heart Rate Control by the Nucleus Ambiguus
IHC-Fr / Mouse
Leptin receptor neurons in the dorsomedial hypothalamus input to the circadian feeding network
IHC-Fr / Mouse
BMSCs Promote Differentiation of Enteric Neural Precursor Cells to Maintain Neuronal Homeostasis in Mice With Enteric Nerve Injury
ICC/IF, IHC-free-floating / Mouse
