ARG62343
anti-GFP antibody [GF28R]
anti-GFP antibody [GF28R] for Dot blot,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [GF28R] recognizes GFP |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, ICC/IF, IP, WB |
| Specificity | Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GF28R |
| Isotype | IgG2b |
| Target Name | GFP |
| Antigen Species | Others |
| Immunogen | GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A Purified |
| Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | In cell and molecular biology, the GFP protein (238aa, 26.9kDa) is frequently used as a reporter to analysis the protein expressing. In modified forms of GFP has been used to be biosensors, and many animals have been created GFP expressing model to analysis gene expression and biological function by transfecting GFP expressing gene into organisms or cells by breeding, injection with a viral vector, or cell transformation. In addition, the GFP gene has been transfected and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein. [Modified from wikipedia] |
| Highlight | Related Antibody Duos and Panels: ARG30001 Tag Internal Control Antibody Duo (GFP, GAPDH) ARG30209 Fluorescent-Tags Antibody Panel Related products: GFP antibodies; GFP ELISA Kits; GFP Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody |
Images (1) Click the Picture to Zoom In
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ARG62343 anti-GFP antibody [GF28R] WB image
Western Blot: 1) HEK293 untransfected control, 2) 1 μg, 3) 10 μg of lysate from GFP-tagged expressing plasmid transfected HEK293 stained with ARG62343 anti-GFP antibody [GF28R] at 1:1000 (1 μg/mL) dilution.
Specific References
Enhancing Efficacy of Albumin-Bound Paclitaxel for Human Lung and Colorectal Cancers through Autophagy Receptor Sequestosome 1 (SQSTM1)/p62-Mediated Nanodrug Delivery and Cancer therapy
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