ARG55727

anti-GFP antibody

anti-GFP antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Overview

Product Description Goat Polyclonal antibody recognizes GFP
Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody does recognize YFP and CFP and does not recognize RFP and mCherry fluorescent proteins.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name GFP
Immunogen Purified recombinant peptide produced in E. coli
Conjugation Un-conjugated
Alternate Names green fluorescent protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:1000
IHC-Fr1:50-1:1000
IHC-P1:50-1:1000
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (2) Click the Picture to Zoom In

  • ARG55727 anti-GFP antibody WB image

    Western blot: 50 µg of HEK293 cell lysates stained with ARG55727 anti-GFP antibody at 1:1000 dilution. CEDOC/FCM - NOVA University of Lisbon, Portugal.

  • ARG55727 anti-GFP antibody ICC/IF image

    Immunocytochemistry: AtT20 transfected with GFP-Rab27a and stained with ARG55727 anti-GFP antibody. Faculty of Medicine/IBILI - University of Coimbra.