ARG62344
anti-RFP antibody [RF5R]
anti-RFP antibody [RF5R] for Dot blot,ELISA,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Other
Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
Overview
| Product Description | Mouse Monoclonal antibody [RF5R] recognizes RFP |
|---|---|
| Tested Reactivity | Other |
| Tested Application | Dot, ELISA, ICC/IF, IHC-Fr, IP, WB |
| Specificity | Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DsRed, mCherry, mOrange, etc |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | RF5R |
| Isotype | IgG1 |
| Target Name | RFP |
| Immunogen | RFP from the Discosoma sp. (sea anemone) N-terminal peptide-KLH conjugates. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | Protein A affinity chromatography from mouse ascites fluid. |
| Buffer | 10mM PBS (pH 7.2) and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function. DsRed (drFP583) is isolated from Discosoma striata and TurboRFP is isolated from Entacmaea quadricolor (Merzlyak et., al 2007), both proteins are Red fluorescent protein and useful to be reportor genes for cell and animal study. |
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| Highlight | Related Antibody Duos and Panels: ARG30209 Fluorescent-Tags Antibody Panel Related products: RFP antibodies; RFP ELISA Kits; RFP Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody |
Images (1) Click the Picture to Zoom In
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ARG62344 anti-RFP antibody [RF5R] WB image
Western Blot: HEK293 cells transfected with RFP-tagged protein vector; (1) untransfected and (2) transfected with Turbo-RFP (2), and (3) transfected with DsRed stained with ARG62344 anti-RFP antibody [RF5R] at 1:1000 (1 μg/mL) dilution
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Review for anti-RFP antibody [RF5R]
Application：IHC
Sample：Drosophila melanogaster, third instar larvae, wing disc
Sample Preparation Method：Third instar larvae were heat-shock at 37ºC for 1 hr. Dissection in 3 day after heat-shock. Disc were fixed in 4% PFA for 20 min.
Fixation Buffer：4% PFA
Fixation Time：20 min
Fixation Temperature：N/A ºC
Antigen Retrieval Buffer：N/A
Permeabilization Buffer：0.1% Triton-X 100
Primary Antibody Dilution Factor：1:250
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
