ARG55744

anti-RFP antibody

anti-RFP antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
publication_link Publication1

Overview

Product Description Goat Polyclonal antibody recognizes RFP
Tested Reactivity Other
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody recognizes red fluorescent protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato. It does not cross-react to GFP.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RFP
Immunogen Purified recombinant RFP peptide produced in E. coli
Conjugation Un-conjugated
Alternate Names Cherry fluorescent protein; dsRed; red fluorescent protein; tdTomato

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:500
IHC-Fr1:50-1:500
IHC-P1:50-1:500
WB1:500-1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (2) Click the Picture to Zoom In

  • ARG55744 anti-RFP antibody WB image

    Western blot: 50 µg of 293HEK cells transduced with RFP Ad stained with ARG55744 anti-RFP antibody at 1:2000 dilution.

  • ARG55744 anti-RFP antibody ICC/IF image

    Immunocytochemistry: 293HEK cells transfected with RFP stained with ARG55744 anti-RFP antibody at 1:50 dilution. Cells were fixed with 4% PFA.

Specific References

C-X-C domain ligand 14-mediated stromal cell-macrophage interaction as a therapeutic target for hand dermal fibrosis

IHC-P / 

Atsushi Goto et al.
Commun Biol.,  (2023)

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