ARG10706
anti-Cas9 (S. pyogenes) antibody [3F9]
anti-Cas9 (S. pyogenes) antibody [3F9] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
Overview
| Product Description | Mouse Monoclonal antibody [3F9] recognizes Cas9 (S. pyogenes) |
|---|---|
| Tested Reactivity | Bacteria |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3F9 |
| Isotype | IgG |
| Target Name | Cas9 (S. pyogenes) |
| Antigen Species | Bacteria |
| Immunogen | N-terminal region, aa. 1-608 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related products: Cas9 antibodies; Anti-Mouse IgG secondary antibodies; Related news: How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer |
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Images (2) Click the Picture to Zoom In
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ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] ICC/IF image
Immunocytochemistry: Transfected HEK293 cells overexpressing the N-terminal aa. 1-608 of S. pyogenes Cas9. The cells were stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] (red), and these cells also co-stained with ARG10734 anti-Cas9 antibody to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most HEK293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.
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ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] WB image
Western blot: 1) MW marker, 2) crude protein extract from Hek293 culture transfected with the immunogen, the N-terminal 1-608 amino acids, 3) Non-transfected control Hek293 cell extract, and 4) 40 ng of Streptococcus pyogenes Cas9 (full length) was stained with ARG10706 anti-Cas9 (S. pyogenes) antibody [3F9] at 1:1000 dilution.
