ARG10760
anti-mCherry antibody
anti-mCherry antibody for ICC/IF,IHC-Frozen sections,Western blot and Other
Overview
| Product Description | Rabbit Polyclonal Antibody to mCherry red fluorescent protein |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Specificity | This antibody also reacts to tdTomato protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | mCherry |
| Antigen Species | Others |
| Immunogen | Full length recombinant protein. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
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ARG10760 anti-mCherry antibody ICC/IF image
Immunocytochemistry: HEK293 cells transfected in the same way and viewed using a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with ARG10760 anti-mCherry antibody is shown in Green. Green antibody staining is only seen in cells which express mCherry, as expected, and the superimposition of the green and red signals results in an orange signal. Interestingly, stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules to release the low molecular weight mCherry fluorochrome.
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ARG10760 anti-mCherry antibody WB image
Western blot: HEK293 cells transfected with pFin-EF1-mCherry vector ("+"), and HEK293 cells which were not transfected with this vector ("-"). The blots were stained with ARG10760 anti-mCherry antibody.
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ARG10760 anti-mCherry antibody WB image
Western blot: 1) HEK293 (non-transfected), 2) HEK293 transfected with mCherry-HA construct, 3) Recombinant mCherry protein, 4) Recombinant GFP protein and 5) HEK293 transfected with a full length GFP construct. The blots were stained with ARG10760 anti-mCherry antibody (green) at 1:1000 dilution. The same blot was simultaneously stained with ARG10757 anti-Hsp 60 antibody (red) at 1:5000 dilution.
