ARG83522
arigoQIKTM Rat IL1 beta ELISA Kit
arigoQIKTM Rat IL1 beta ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG83522-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83522-02 | Standard | 2 vials | 4°C |
| ARG83522-03 | 1000X HRP-Streptavidin solution | 15 μl | 4°C |
| ARG83522-04 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG83522-05 | HRP Diluent Buffer | 20 ml | 4°C |
| ARG83522-06 | 10X Wash Buffer | 35 ml | 4°C |
| ARG83522-07 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83522-08 | STOP solution | 12 ml | 4°C |
| ARG83522-09 | Plate sealer | 2 strips | Room temperature |
Overview
| Product Description | ARG83522 arigoQIKTM Rat IL1 betaELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat IL1 beta in serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | IL1 beta |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 15.63 - 1000 pg/ml |
| Sample Volume | 50 μL |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | IL1B; Interleukin 1 Beta; IL1F2 2; IL1-BETA; Interleukin-1 Beta; IL-1 Beta; Catabolin; IL-1B; Pro-Interleukin-1-Beta; Preinterleukin 1 Beta; Interleukin 1beta; IL1beta; IL-1 |
Application Instructions
| Assay Time | 2 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C, -80°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL1B |
|---|---|
| Gene Full Name | interleukin 1, beta |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020] |
| Function | Acts as a sensor of S.pyogenes infection in skin: cleaved and activated by pyogenes SpeB protease, leading to an inflammatory response that prevents bacterial growth during invasive skin infection. [Uniprot] |
| Cellular Localization | Cytoplasm, Lysosome, Secreted. [Uniprot] |
Images (1) Click the Picture to Zoom In
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ARG83522 arigoQIKTM Rat IL1 beta ELISA Kit standard curve image
ARG83522 arigoQIKTM Rat IL1 beta ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG83522 arigoQIKTM Rat IL1 beta ELISA Kit User manual |
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