ARG83521
arigoQIKTM Mouse VEGF ELISA Kit
arigoQIKTM Mouse VEGF ELISA Kit for ELISA and Mouse
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG83521-01 | Antibody Coated Microplate | 8 X 12 strips | 4°C |
| ARG83521-02 | Standard | 2 vials | 4°C |
| ARG83521-03 | 1000X HRP-Streptavidin solution | 15 μl | 4°C |
| ARG83521-04 | Standard/Sample Diluent Buffer | 20 ml | 4°C |
| ARG83521-05 | HRP Diluent Buffer | 20 ml | 4°C |
| ARG83521-06 | 10X Wash Buffer | 35 ml | 4°C |
| ARG83521-07 | TMB substrate | 12 ml | 4°C (protect from light) |
| ARG83521-08 | STOP solution | 12 ml | 4°C |
| ARG83521-09 | Plate sealer | 2 strips | Room temperature |
Overview
| Product Description | ARG83521 arigoQIKTMMouse VEGF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse VEGF serum, plasma and cell culture supernatants. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA |
| Target Name | VEGF |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 11.71 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 23.44 - 1500 pg/ml |
| Sample Volume | 50 μL |
| Precision | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| Alternate Names | EGFA; Vascular Endothelial Growth Factor A; VPF; VEGF; Vascular Endothelial Growth Factor A, Long Form; Vascular Permeability Factor; VEGF-A; L-VEGF; Vascular Endothelial Growth Factor A121; Vascular Endothelial Growth Factor A165; Vascular Endothelial Growth Factor; MVCD1 |
Application Instructions
| Assay Time | 2 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 4°C, -20°C, -80°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q00731 Mouse Vascular endothelial growth factor A |
|---|---|
| Gene Symbol | Vascular Endothelial Growth Factor A |
| Gene Full Name | VEGFA |
| Background | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces prolifeMouseion and migMouseion of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiMouseory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. [provided by RefSeq, Jun 2020] |
| Function | Induces endothelial cell prolifeMouseion, promotes cell migMouseion, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migMouseion, including for the caudal migMouseion of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. [Uniprot] |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum, Extracellular matrix, Golgi appaMouseus, Nucleus, Secreted. [Uniprot] |
Images (1) Click the Picture to Zoom In
-
ARG83521 arigoQIKTM Mouse VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG83521 arigoQIKTM Mouse VEGF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| arigoQIKTM Mouse VEGF ELISA Kit User manual |
 Download
|
