ARG59671
anti-vWF antibody
anti-vWF antibody for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes vWF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | vWF |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to R2535-K2813 of Human vWF. |
| Conjugation | Un-conjugated |
| Alternate Names | VWD; von Willebrand factor; vWF; von Willebrand antigen II; F8VWF |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VWF |
| Gene Full Name | von Willebrand factor |
| Background | The glycoprotein encoded by this gene functions as both an antihemophilic factor carrier and a platelet-vessel wall mediator in the blood coagulation system. It is crucial to the hemostasis process. Mutations in this gene or deficiencies in this protein result in von Willebrand's disease. An unprocessed pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008] |
| Function | Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. [UniProt] |
| Cellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules. [UniProt] |
| Calculated MW | 309 kDa |
| PTM | All cysteine residues are involved in intrachain or interchain disulfide bonds. N- and O-glycosylated. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG59671 anti-vWF antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59671 anti-vWF antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59671 anti-vWF antibody WB image
Western blot: 50 µg of sample under reducing conditions. HT1080 whole cell lysate stained with ARG59671 anti-vWF antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59671 anti-vWF antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59671 anti-vWF antibody (blue line) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green line) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red line) was also used as a control.
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ARG59671 anti-vWF antibody IHC-Fr image
Immunohistochemistry: Frozen section of Human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59671 anti-vWF antibody at 1 µg/ml dilution, overnight at 4°C.
