ARG10045

anti-serum Amyloid A antibody [115]

anti-serum Amyloid A antibody [115] for ELISA,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody

Overview

Product Description

Mouse Monoclonal antibody [115] recognizes serum Amyloid A

Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, TGF-β and EGF.
Host Mouse
Clonality Monoclonal
Clone 115
Isotype IgG1, kappa
Target Name serum Amyloid A
Antigen Species Human
Immunogen Highly purified recombinant human serum Amyloid A (MW: 12 kDa)
Conjugation Un-conjugated
Alternate Names 2-104; 2-103; 2-102; Serum amyloid A-1 protein; TP53I4; SAA; Amyloid fibril protein AA; SAA2; PIG4; 4-101; 3-104

Application Instructions

Application Note ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human SAA detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10205). Suggested capture coating dose: 0.3μg/well.

Western Blot: The antibody, when used at concentration of 0.1-0.5μg/mL will allow visualization of 100 ng/lane of recombinant human SAA.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6288 Human SAA1

Swiss-port # P0DJI8 Human Serum amyloid A-1 protein

Gene Symbol SAA1
Gene Full Name serum amyloid A1
Background This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]
Function Major acute phase protein. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30077 serum Amyloid A ELISA Antibody Duo
Related products:
serum Amyloid A antibodies; serum Amyloid A ELISA Kits; serum Amyloid A Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody
Calculated MW 14 kDa
PTM This protein is the precursor of amyloid protein A, which is formed by the removal of approximately 24 residues from the C-terminal end.

Clone References

Comparative proteomic analysis in children with idiopathic short stature (ISS) before and after short-term recombinant human growth hormone (rhGH) therapy.

Heo SH et al.
Proteomics.,  (2013)

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Elevation of intact and proteolytic fragments of acute phase proteins constitutes the earliest systemic antiviral response in HIV-1 infection.

WB / Human

Kramer HB et al.
PLoS Pathog.,  (2010)

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Serum amyloid A inhibits apoptosis of human neutrophils via a P2X7-sensitive pathway independent of formyl peptide receptor-like 1.

Christenson K et al.
J Leukoc Biol.,  (2008)

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Inflammatory protein profile during systemic high dose interleukin-2 administration.

Rossi L et al.
Proteomics.,  (2006)

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