ARG45466
anti-p95 / NBS1 antibody [5D7A1]
anti-p95 / NBS1 antibody [5D7A1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [5D7A1] recognizes p95 / NBS1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5D7A1 |
| Isotype | IgG2a |
| Target Name | p95 / NBS1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminal region of human p95 / NBS1. |
| Conjugation | Un-conjugated |
| Alternate Names | NBN; Nibrin; AT-V2; AT-V1; NBS1; ATV; NBS; Nijmegen Breakage Syndrome 1 (Nibrin); Cell Cycle Regulatory Protein P95; HNbs1; P95; P95 Protein Of The MRE11/RAD50 Complex; Nijmegen Breakage Syndrome Protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 95 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NBN |
| Gene Full Name | Nibrin |
| Background | Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008] |
| Function | Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. [UniProt] |
| Cellular Localization | Chromosome; Nucleus; Telomere. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | Isopeptide bond; Phosphoprotein; Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] IHC-P image
Immunohistochemistry: Human testis cancer stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 2 μg/ml dilution.
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] ICC/IF image
Immunofluorescence: A431 stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 5 μg/ml dilution.
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] WB image
Western blot: A549 stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 0.5 μg/ml dilution.
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] FACS image
Flow Cytometry: A431 stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 1 µg/10^6 cells dilution.
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] WB image
Western blot: Rat testis stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 0.5 μg/ml dilution.
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ARG45466 anti-p95 / NBS1 antibody [5D7A1] WB image
Western blot: Mouse brain stained with ARG45466 anti-p95 / NBS1 antibody [5D7A1] at 0.5 μg/ml dilution.
