ARG57483

anti-p70 S6 Kinase antibody

anti-p70 S6 Kinase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes p70 S6 Kinase
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Specificity This antibody detects endogenous levels of total p70 S6 Kinase.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name p70 S6 Kinase
Antigen Species Human
Immunogen Synthetic peptide derived from Human p70 S6 Kinase.
Conjugation Un-conjugated
Alternate Names S6K-beta2; p70 ribosomal S6 kinase beta; Ribosomal protein S6 kinase beta-2; S6 kinase-related kinase; S6K-beta-2; p70-beta; p70 S6K-beta; p70 S6 kinase beta; p70S6Kb; STK14B; p70 S6KB; p70-S6K 2; P70-beta-1; P70-beta-2; S6K2; SRK; P70S6K2; KLS; Serine/threonine-protein kinase 14B; S6K-beta; P70-beta; p70(S6K)-beta; 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:100 - 1:500
IHC-P1:50 - 1:200
IP1:50
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 58988 Mouse RPS6KB2

GeneID: 6199 Human RPS6KB2

Swiss-port # Q9UBS0 Human Ribosomal protein S6 kinase beta-2

Swiss-port # Q9Z1M4 Mouse Ribosomal protein S6 kinase beta-2

Gene Symbol RPS6KB2
Gene Full Name ribosomal protein S6 kinase, 70kDa, polypeptide 2
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015]
Function Phosphorylates specifically ribosomal protein S6. [UniProt]
Calculated MW 53 kDa
PTM Phosphorylated and activated by MTOR. Phosphorylation by PKC within the NLS in response to mitogenic stimuli causes cytoplasmic retention.

Images (1) Click the Picture to Zoom In

  • ARG57483 anti-p70 S6 Kinase antibody WB image

    Western blot: 293T cell lysate stained with ARG57483 anti-p70 S6 Kinase antibody.