ARG52339
anti-mGluR1a + mGluR5 antibody
anti-mGluR1a + mGluR5 antibody for IHC-Frozen sections,Western blot and Rat
Cancer antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes mGluR1a + mGluR5 |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms |
| Tested Application | IHC-Fr, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | mGluR1a + mGluR5 |
| Antigen Species | Rat |
| Immunogen | Peptide from the C-terminal region of rat mGluR5 and rat mGluR1a. |
| Conjugation | Un-conjugated |
| Alternate Names | PPP1R86; mGlu5; GPRC1E; Metabotropic glutamate receptor 5; mGluR5; MGLUR5 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~125k monomer and the ~250k dimers of mGluR5 and mGluR1. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to geneRate the antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GRM5 |
| Gene Full Name | glutamate receptor, metabotropic 5 |
| Background | The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004). |
| Research Area | Cancer antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 132 kDa |
Images (1) Click the Picture to Zoom In
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ARG52339 anti-mGluR1a + mGluR5 antibody WB image
Western blot: 10 μg of HEK 293 cells expressing mGluR1a and mGluR5 showing specific immunolabeling of the ~125k monomer and the ~250k dimers of mGluR1a and mGluR5 stained with ARG52339 anti-mGluR1a + mGluR5 antibody.
