ARG20525
anti-eNOS phospho (Ser632) antibody [M232]
anti-eNOS phospho (Ser632) antibody [M232] for ELISA,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [M232] recognizes eNOS phospho (Ser632) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, WB |
| Specificity | The antibody detects a ~140 and 120 kDa bands on SDS-PAGE immunoblots of human umbilical vein endothelial cells, but these bands are not observed after lambda phosphatase treatment. The 120 kDa band may be a truncated form of eNOS. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M232 |
| Isotype | IgG1 |
| Target Name | eNOS |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide (coupled to carrier protein) around Ser632 of Mouse eNOS. This sequence is conserved in human (Ser-633) and rat (Ser-632) eNOS, and has low homology to other NOS family members. |
| Conjugation | Un-conjugated |
| Alternate Names | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Nos3 |
| Gene Full Name | nitric oxide synthase 3, endothelial cell |
| Background | Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 133 kDa |
| PTM | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. |
Images (1) Click the Picture to Zoom In
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ARG20525 anti-eNOS phospho (Ser632) antibody [M232] WB image
Western blot: 1) and 2) calyculin A (100 nM) treated Human umbilical vein endothelial cells for 30 min, 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20525 anti-eNOS phospho (Ser632) antibody [M232]. The antibody detects eNOS phospho (Ser632) at around 140 kda, and the 100 kda signal might be a non-specific signal.
