ARG67130
anti-eNOS phospho (Ser1177) antibody
anti-eNOS phospho (Ser1177) antibody for ELISA,ICC/IF,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes eNOS phospho (Ser1177) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | The antibody detects a ~140 kDa band corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells grown normally or treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | eNOS |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (coupled to carrier protein) around Ser1177 of Human eNOS. This sequence is conserved in rat and mouse eNOS. |
| Conjugation | Un-conjugated |
| Alternate Names | NOS3; Nitric Oxide Synthase 3; ENOS; ECNOS; Nitric Oxide Synthase 3 (Endothelial Cell); Constitutive NOS; Endothelial NOS; EC 1.14.13.39; NOS Type III; EC-NOS; NOSIII; CNOS; Nitric Oxide Synthase, Endothelial; Endothelial Nitric Oxide Synthase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | PBS, 0.02% Sodium azide, 0.5% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA, 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NOS3 |
| Gene Full Name | Nitric Oxide Synthase 3 |
| Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Function | NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. [UniProt] |
| Cellular Localization | Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30247 Phospho eNOS Antibody Duo (Total, pY1177) Related products: eNOS antibodies; eNOS ELISA Kits; eNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 133 kDa |
| PTM | Lipoprotein, Myristate, Palmitate, Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG67130 anti-eNOS phospho (Ser1177) antibody ICC/IF image
Immunofluorescence: A549 stained with ARG67130 anti-eNOS phospho (Ser1177) antibody at 1:200 dilution.
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ARG67130 anti-eNOS phospho (Ser1177) antibody WB image
Western blot: HuvEc stained with ARG67130 anti-eNOS phospho (Ser1177) antibody at 1:1000 dilution.
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ARG67130 anti-eNOS phospho (Ser1177) antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG67130 anti-eNOS phospho (Ser1177) antibody at 1:200 dilution.
