ARG23708
anti-eNOS antibody [M221]
anti-eNOS antibody [M221] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [M221] recognizes eNOS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M221 |
| Isotype | IgG1 |
| Target Name | eNOS |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein around the C-terminus region of Mouse eNOS. This sequence is conserved in Human and Rat eNOS, and has low homology to other NOS family members. |
| Conjugation | Un-conjugated |
| Alternate Names | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. IHC-P: Antigen Retrieval: Heat mediation was performed in 10 mM Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NOS3 |
| Gene Full Name | nitric oxide synthase 3 (endothelial cell) |
| Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| Calculated MW | 133 kDa |
| PTM | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG23708 anti-eNOS antibody [M221] WB image
Western blot: Human umbilical vein endothelial cells untreated (left) or treated (right) with lambda phosphatase. The blots were stained with ARG23708 anti-eNOS antibody [M221].
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ARG23708 anti-eNOS antibody [M221] ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed and NP-40-permeabilized Human umbilical vein endothelial cells. The cells were stained with ARG23708 anti-eNOS antibody [M221].
