Overview

Product Description	Rabbit Polyclonal antibody recognizes eIF4E2
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	eIF4E2
Antigen Species	Human
Immunogen	Recombinant protein of Human eIF4E2.
Conjugation	Un-conjugated
Alternate Names	Eukaryotic translation initiation factor 4E homologous protein; mRNA cap-binding protein type 3; EIF4EL3; IF4e; Eukaryotic translation initiation factor 4E type 2; 4E-LP; eIF4E-like protein 4E-LP; eIF-4E type 2; mRNA cap-binding protein 4EHP; eIF4E type 2; Eukaryotic translation initiation factor 4E-like 3; 4EHP

Application Instructions

Predict Reactivity Note	Rat
Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:200 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HL-60
Observed Size	~ 30 kDa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 26987 Mouse EIF4E2 GeneID: 9470 Human EIF4E2 Swiss-port # O60573 Human Eukaryotic translation initiation factor 4E type 2 Swiss-port # Q8BMB3 Mouse Eukaryotic translation initiation factor 4E type 2
Gene Symbol	EIF4E2
Gene Full Name	eukaryotic translation initiation factor 4E family member 2
Function	Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. [UniProt]
Calculated MW	28 kDa
PTM	Ubiquitinated by ARIH1 (PubMed:14623119, PubMed:25624349). The consequences of ubiquitination are however unclear: according to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report ubiquitination leads to its subsequent degradation (PubMed:14623119). ISGylation enhances its cap structure-binding activity and translation-inhibition activity.

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