ARG57332

anti-eIF4A1 antibody

anti-eIF4A1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes eIF4A1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Specificity This antibody might also react to eIF4A2 based on sequence analysis (90%).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eIF4A1
Antigen Species Human
Immunogen Recombinant Protein of Human eIF4A1.
Conjugation Un-conjugated
Alternate Names ATP-dependent RNA helicase eIF4A-1; EIF4A; Eukaryotic initiation factor 4A-I; EIF-4A; DDX2A; EC 3.6.4.13; eIF4A-I; eIF-4A-I

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IP1:20 - 1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13681 Mouse EIF4A1

GeneID: 1973 Human EIF4A1

Swiss-port # P60842 Human Eukaryotic initiation factor 4A-I

Swiss-port # P60843 Mouse Eukaryotic initiation factor 4A-I

Gene Symbol EIF4A1
Gene Full Name eukaryotic translation initiation factor 4A1
Function ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. [UniProt]
Calculated MW 46 kDa

Images (2) Click the Picture to Zoom In

  • ARG57332 anti-eIF4A1 antibody ICC/IF image

    Immunofluorescence: MCF-7 cells stained with ARG57332 anti-eIF4A1 antibody.

  • ARG57332 anti-eIF4A1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG57332 anti-eIF4A1 antibody.